新鲜、玻璃化冷冻牛卵母细胞体外受精囊胚全基因组甲基化模式初探

doi:10.11843/j.issn.0366-6964.2019.06.008

Abstract

Abstract: The aim of this study was to analyze the genomic methylation patterns of in vitro fertilized(IVF) blastocysts from fresh and vitrified bovine oocytes. In this study, the whole-genome methylation level and differential methylation regions of in vitro fertilized(IVF) blastocysts from fresh and vitrified bovine oocytes were detected by single-cell whole-genome methylation sequencing technique(scWGMS). The difference of DNA methylation level between in vitro fertilized(IVF) blastocysts from fresh and vitrified bovine oocytes was investigated. The results showed that the whole methylation level of in vitro fertilized(IVF) blastocysts from fresh oocytes was significantly higher than that of in vitro fertilized(IVF) blastocysts from vitrified oocytes in bovine(P<0.05). GO and KEGG were used to analyze 143 DMRs. It was found that biological process of these DMRs were mainly involved in metabolism, growth and development, cell localization and response to cell stimulation. The pathways were mainly enriched in growth and development, nucleic acid binding and histone acetylation, and the partial related candidate genes were screened(FARP2, PI4KA, FAM3D, NCOR2, ZNF827, etc.). In this study, we found that the whole genome methylation level of in vitro fertilized(IVF) blastocysts from vitrified bovine oocytes was significantly decreased, and the DMR region was mainly involved in ATP binding, growth and development and histone acetylation, which provided information reference for improving the quality of IVF blastocysts from vitrified bovine oocytes.

CLC Number:

S823.3

ZHAO Yahan, HAO Haisheng, DU Weihua, PANG Yunwei, YAN Changliang, LIU Yan, ZHAO Shanjiang, ZHAO Xueming, ZHU Huabin. Study on Whole Genome Methylation Pattern of in vitro Fertilized Blastocysts from Fresh and Vitrified Bovine Oocytes[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(6): 1179-1188.

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Study on Whole Genome Methylation Pattern of in vitro Fertilized Blastocysts from Fresh and Vitrified Bovine Oocytes

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